"We are not ready to surrender, and will never be ready to surrender, the independence of our foreign policy", he said.
After initially taking credit for the moves to isolate Qatar ("Perhaps this will be the beginning of the end to the horror of terrorism!" the president tweeted Tuesday), Trump called Qatar's leader Wednesday with an offer to help Gulf countries resolve the growing diplomatic crisis, through a meeting at the White House if necessary.
Although it has been widely known that Saudi Arabia, Egypt, the UAE and Bahrain have always been frustrated by Doha's foreign policy activities that have shown support for the Muslim Brotherhood in Egypt, Islamist factions in Syria and friendly relations with Iran, the severity of the blockade reveals a much deeper mistrust than had previously been realised.
The Gulf countries have ordered their citizens out of Qatar and gave Qataris overseas 14 days to return home.
Qatar is also home to the biggest American air base in the region.
Saudi Arabia's King Salman bin Abdulaziz Al Saud (R) met with Bahrain's King Hamad bin Isa Al Khalifa in Jeddah, on Wednesday. Kuwait's ruler, meanwhile, is making an effort to help mediate. As relationship dynamics in the Middle East shift, we asked professor Denis Sullivan, co-director of the Middle East Center at Northeastern, to explain the geopolitical impact of isolating Qatar and how the USA might respond.
While the attack maybe - and probably is - the sole effort of IS which is battling against Iran backed groups in Iraq and Syria, in light of recent events it is likely that narratives of a Saudi-US conspiracy against will find traction in Iran, especially among its zealous political parties.
His views were echoed by Kristian Coates Ulrichsen, fellow for the Middle East at the Baker Institute for Public Policy at Rice University, who said that the branch campuses would inevitably be affected if Qatar remained under prolonged economic sanctions.
A clearly furious Iran Foreign Minister Javad Zarif responded on Twitter Thursday, describing Trump's statement as "repugnant". The agreement did not detail a timeframe or the number of troops.
Analysts say the crisis is in part an extension of a 2014 dispute, when Saudi Arabia, the UAE and Bahrain temporarily recalled their ambassadors from Doha over Qatari support for Egypt's Muslim Brotherhood. "We need the world to condemn the aggressors".
"The nation of Qatar, unfortunately, has historically been a funder of terrorism at a very high level", Trump said, echoing an allegation the Saudi-led group has used to justify cutting diplomatic ties to the tiny gas-rich kingdom.
The UAE's central bank also ordered the country's financial institutions to apply enhanced scrutiny on any accounts with six Qatari banks, including Qatar National Bank, which is the largest bank in Africa and the Middle East, and could potentially affect liquidity in the Qatari banking system.
Gargash said "not using the formidable media ownership in promoting an extremist agenda" was a condition for negotiations.